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keith shearwin  (Addgene inc)


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    Structured Review

    Addgene inc keith shearwin
    Keith Shearwin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/keith+shearwin/pE-FLP+(Plasmid+%2345978)/pm41529903-150-50-52
    Average 94 stars, based on 27 article reviews
    keith shearwin - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: SHARK: A Specialized Host for Assembling R6K Plasmids.
    Article Snippet: .. The λRED operon and recA were taken from pREDCas9, a gift from Tao Chen (Addgene plasmid No.71541);43 the salicylic acid inducible promoter and transcription factor were from pAJM.771, a gift from Christopher Voigt (Addgene plasmid No.108534),11 and the pSC101TS backbone was from pE-FLP, a gift from from Drew Endy and Keith Shearwin (Addgene plasmid No. 45978).4 pREMORAv1 was constructed using Golden-Gate cloning and was assembled in two steps; first the λRED operon and PlexA-recA gene were placed under PSalTTC regulation on pAJM.771, this plasmid has a p15A-Kanamycin backbone. ..

    Article Title: SHARK: A Specialized Host for Assembling R6K Plasmids
    Article Snippet: .. The λRED operon and recA were taken from pREDCas9, a gift from Tao Chen (Addgene plasmid No.71541); the salicylic acid inducible promoter and transcription factor were from pAJM.771, a gift from Christopher Voigt (Addgene plasmid No.108534), and the pSC101TS backbone was from pE-FLP, a gift from from Drew Endy and Keith Shearwin (Addgene plasmid No. 45978). pREMORAv1 was constructed using Golden-Gate cloning and was assembled in two steps; first the λRED operon and P lexA - recA gene were placed under PSalTTC regulation on pAJM.771, this plasmid has a p15A-Kanamycin backbone. ..

    Article Title: SHARK: a specialized host for assembling R6K plasmids
    Article Snippet: .. The λ RED operon and recA were taken from pREDCas9, a gift from Tao Chen (Addgene plasmid No.71541) [ ]; the salicylic acid inducible promoter and transcription factor were from pAJM.771, a gift from Christopher Voigt (Addgene plasmid No.108534) [ ], and the pSC101TS backbone was from pE-FLP, a gift from from Drew Endy and Keith Shearwin (Addgene plasmid No. 45978) [ ]. pREMORA was constructed using Golden-gate cloning, and was assembled in two steps; first the λ RED operon and P lexA - recA gene were placed under PSalTC regulation on pAJM.771, this plasmid has a p15A-Kanamycin backbone. ..

    Construct:

    Article Title: SHARK: A Specialized Host for Assembling R6K Plasmids.
    Article Snippet: .. The λRED operon and recA were taken from pREDCas9, a gift from Tao Chen (Addgene plasmid No.71541);43 the salicylic acid inducible promoter and transcription factor were from pAJM.771, a gift from Christopher Voigt (Addgene plasmid No.108534),11 and the pSC101TS backbone was from pE-FLP, a gift from from Drew Endy and Keith Shearwin (Addgene plasmid No. 45978).4 pREMORAv1 was constructed using Golden-Gate cloning and was assembled in two steps; first the λRED operon and PlexA-recA gene were placed under PSalTTC regulation on pAJM.771, this plasmid has a p15A-Kanamycin backbone. ..

    Article Title: SHARK: A Specialized Host for Assembling R6K Plasmids
    Article Snippet: .. The λRED operon and recA were taken from pREDCas9, a gift from Tao Chen (Addgene plasmid No.71541); the salicylic acid inducible promoter and transcription factor were from pAJM.771, a gift from Christopher Voigt (Addgene plasmid No.108534), and the pSC101TS backbone was from pE-FLP, a gift from from Drew Endy and Keith Shearwin (Addgene plasmid No. 45978). pREMORAv1 was constructed using Golden-Gate cloning and was assembled in two steps; first the λRED operon and P lexA - recA gene were placed under PSalTTC regulation on pAJM.771, this plasmid has a p15A-Kanamycin backbone. ..

    Article Title: SHARK: a specialized host for assembling R6K plasmids
    Article Snippet: .. The λ RED operon and recA were taken from pREDCas9, a gift from Tao Chen (Addgene plasmid No.71541) [ ]; the salicylic acid inducible promoter and transcription factor were from pAJM.771, a gift from Christopher Voigt (Addgene plasmid No.108534) [ ], and the pSC101TS backbone was from pE-FLP, a gift from from Drew Endy and Keith Shearwin (Addgene plasmid No. 45978) [ ]. pREMORA was constructed using Golden-gate cloning, and was assembled in two steps; first the λ RED operon and P lexA - recA gene were placed under PSalTC regulation on pAJM.771, this plasmid has a p15A-Kanamycin backbone. ..

    Cloning:

    Article Title: SHARK: A Specialized Host for Assembling R6K Plasmids.
    Article Snippet: .. The λRED operon and recA were taken from pREDCas9, a gift from Tao Chen (Addgene plasmid No.71541);43 the salicylic acid inducible promoter and transcription factor were from pAJM.771, a gift from Christopher Voigt (Addgene plasmid No.108534),11 and the pSC101TS backbone was from pE-FLP, a gift from from Drew Endy and Keith Shearwin (Addgene plasmid No. 45978).4 pREMORAv1 was constructed using Golden-Gate cloning and was assembled in two steps; first the λRED operon and PlexA-recA gene were placed under PSalTTC regulation on pAJM.771, this plasmid has a p15A-Kanamycin backbone. ..

    Article Title: SHARK: A Specialized Host for Assembling R6K Plasmids
    Article Snippet: .. The λRED operon and recA were taken from pREDCas9, a gift from Tao Chen (Addgene plasmid No.71541); the salicylic acid inducible promoter and transcription factor were from pAJM.771, a gift from Christopher Voigt (Addgene plasmid No.108534), and the pSC101TS backbone was from pE-FLP, a gift from from Drew Endy and Keith Shearwin (Addgene plasmid No. 45978). pREMORAv1 was constructed using Golden-Gate cloning and was assembled in two steps; first the λRED operon and P lexA - recA gene were placed under PSalTTC regulation on pAJM.771, this plasmid has a p15A-Kanamycin backbone. ..

    Article Title: SHARK: a specialized host for assembling R6K plasmids
    Article Snippet: .. The λ RED operon and recA were taken from pREDCas9, a gift from Tao Chen (Addgene plasmid No.71541) [ ]; the salicylic acid inducible promoter and transcription factor were from pAJM.771, a gift from Christopher Voigt (Addgene plasmid No.108534) [ ], and the pSC101TS backbone was from pE-FLP, a gift from from Drew Endy and Keith Shearwin (Addgene plasmid No. 45978) [ ]. pREMORA was constructed using Golden-gate cloning, and was assembled in two steps; first the λ RED operon and P lexA - recA gene were placed under PSalTC regulation on pAJM.771, this plasmid has a p15A-Kanamycin backbone. ..

    other:

    Article Title: CRISPR interference of nucleotide biosynthesis improves production of a single-domain antibody in Escherichia coli.
    Article Snippet: The pOSIP KO and the PE‐FLP plasmids were a gift from Drew Endy and Keith Shearwin (Addgene plasmids 45985 and 45978, respectively).



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